Microarray Based Assays

A rapid oligonucleotide microarray hybridization assay for the discrimination of the six species of the Listeria genus has been developed. The approach involves one-tube multiplex PCR of six target virulence factor genes (iap, hlyA, inlB, plcA, plcB, and clpE), synthesis of fluorescently labeled single-stranded DNA with primer extension of DNA synthesized in the first round of multiplex PCR, and hybridization to the multiple individual oligonucleotide probes specific for each Listeria spe cies and immobilized on a glass surface. This microarray technique was proven to correctly identify all Listeria

species.1 ]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment