Molecular Diagnosis Of Fshd

FSHD is associated with large deletions of the polymorphic D4Z4 repeat array when located at 4q35. The introduction of differential EcoRI/Blnl double digest has led to a dramatic improvement in molecular diagnostics for FSHD.[14,15] The specificity and sensitivity of this EcoRI-Blnl double-digest technique for accurate FSHD diagnosis have been well assessed and demonstrated to be high. However, an additional diagnostic complication is created by subtelomeric chromosomal exchanges between the two highly homologous 4q35 and 10q26 D4Z4 repeat loci, which are present in about 20% of the normal population.[11] Diagnostic complexity has been further increased by the recent observation that these subtelo-meric exchanges may also result in the formation of hybrid arrays, containing an admixture of 4q35-derived and 10q26-derived D4Z4 repeat units. In addition, the complete loss of the D4F104S1 locus (probe p13E-11) has been reported in FSHD patients. Subtelomeric exchanges may lead to problems during the molecular diagnosis of FSHD in 5% of cases.[11,15] Recent findings using XapI, an additional restriction enzyme, have increased the accuracy of molecular diagnosis for FSHD to 98%.[16]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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