Molecular Genetic Testing Dm1

Small expansions are determined by standard PCR. Large expansions are refractory to PCR and are traditionally identified by Southern blotting by hybridization of restriction-digested genomic DNA. For Southern blotting, genomic DNA is digested with EcoRl and then separated according to size by agarose gel electrophoresis. After denaturation and neutralization, DNA fragments are transferred onto a membrane, which is then randomly probed with 32P-labelled cDNA25. Blots are then washed and exposed at — 80°C for 5-10 days.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment