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pAZ102-E 5'-GATGGCTGTTTCCAAGCCCA-3 ' pAZ102-H 5'-GTGTACGTTGCAAAGTACTC-3' pAZ102-L2 5'-ATAAGGTAGATAGTCGAAAG-3' pAZ112-10F 5'-GGGAATTCTAGACGGTCACAGAGATCAG-3' pAZ112-10R 5'-GGGAATTCGAACGATTACTAGCAATTCC-3'

JKK14/15 5'-GAA TGC AAA TCY TTA CAG ACA ACA G-3' JKK17 5'-AAA TCA TGA ACG AAA TAA CCA TTG C-3' PCMSGFRET1U 5'-CAA AAA TAA CAY TSA CAT CAA CRA GGC G-3'

PCMSGFRET1D 5'-TGC AAA CCA ACC AAG TGT ACG ACA GG-3'

950C/1O min [940C/15 sec, 72-62OC/30 sec (-TC /cycle)] x 10 (920C/15 sec, 62OC/30 sec, 720C/15 sec) x 40, 720C/5 min 950C/1O min [940C/15 sec, 65-55OC/30 sec (-TC /cycle), 72OC/60 sec] x 10 (920C/15 sec, 55OC/30 sec, 72OC/60 sec) x 40, 720C/5 min

950C/1O min [950C/5 sec, 65-600C/10 sec (-TC /cycle), 720C/20 sec] x 6 [950C/5 sec, 58-500C/10 sec (-20C /cycle), 720C/20 sec] x 5 (950C/5 sec, 500C/10 sec, 720C/20 sec) x 35

great impact on our understanding of the organism itself 10. and of PCP.

The main fields of research that have benefited from the development of these techniques include characterization of the basic biology of the organism, biology and . epidemiology of PCP, and diagnosis of PCP. Although much is still to be learned about this organism and PCP, it is likely that molecular approaches will continue to be among the most important tools available. The ongoing 12. project to sequence the genome will, when finished, provide important information for investigators conducting research on Pneumocystis.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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