Multiplex Nasba And

Greijer et al.[5] designed a multiplex real-time NASBA using molecular beacon probes labeled with three different fluorophores to quantify human cytomegalovirus (CMV) IE1 mRNA by competitive coamplification of wild-type and calibrator RNA. A simultaneous detection of late pp67mRNA in whole-blood samples of CMV-infected lung transplant patients was also obtained. Despite the somewhat lower sensitivity of the real-time NASBA compared with the conventional NASBA, the simultaneous quantification of IE1 and detection of pp67 RNA was reproducible and accurate. Loens et al.[6] also developed a multiplex real-time NASBA for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila RNA in respiratory specimens by using three fluorophores. They also found a slightly lower sensitivity of the multiplex real-time assay compared to the conventional ECL detection on spiked respiratory samples.

The Procleix human immunodeficiency virus-1/hepa-titis C virus (HIV-1/HCV) assay (Gen-Probe Inc.) detects both HIV- and HCV RNA in 1 tube.[8] The source of the reactivity can be determined by discriminatory assays using virus-specific probes. The HIV-1/HCV assay and the discriminatory assays are able to detect 10-13 copies of HIV-1/mL with 95% detection rates, and can detect 30 copies/mL of HCV with 95% sensitivity. Furthermore, sense RNA VWW> oligo P1

Reverse Transcriptase

Reverse Transcriptase Reverse Transcriptase

RNase H primer P2

Reverse Transcriptase

T7 RNA polymerase é

primer P2

T7 RNA polymerase anti-sense RNA

Fig. 1 NASBA amplification.

the assay can be used with high sensitivity and specificity for the screening of donor blood.[9]

Gaydos et al.[10] evaluated the performance of the APTIMA Combo 2 assay (Gen-Probe Inc.) for the simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae in endocervical swabs and firstcatch urine specimens (FCU) from females, and compared the results with patient infected status, available by using other commercial nucleic acid amplification techniques. Sensitivity and specificity rates for C. trachomatis in swabs were 94.2% and 97.6%, respectively, and 94.7% and 98.9%, respectively, in FCU. Sensitivity and specificity for N. gonorrhoeae in swabs were 99.2% and 98.7%, respectively, and in FCU, 91.3% and 99.3%, respectively.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment