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The detection of NPY mRNA expression in neuroblas-tomas has been of particular interest because NPY is

The recent studies that have used real-time PCR for the quantitation of target genes in neuroblastoma tissues and for the detection of MRD provide encouraging data for the use of this technique introduced only 7 years ago. Apart from a high degree of precision, practical advantages of real-time PCR, such as easy handling, rapid measurements, and a broad linear range for the measurements, have to be highlighted.

From a diagnostic point of view, one interesting aspect for the future might be the use of semiautomated or automated real-time devices for the assessment of gene expression and amplification in neuroblastomas, putting into consideration the relatively easy and little time-consuming way of measurement. Real-time PCR might help to quantify more prognostic markers, expression of genes involved in multidrug resistance, and genes related to tumor invasion and metastasis. There are first reports on the use of multiplex real-time PCR, a development that might certainly facilitate diagnostic procedure in neuro-blastoma diagnostics in the coming years.

Disease management as such might initially not be altered by a new method for the determination of genetic neuroblastoma markers. However, if this relatively novel method becomes available more widely (e.g., as a consequence of dropping costs for this still-very-expensive technique), the number of prognostic markers examined might help to improve therapeutic strategies in the long run.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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