gold standard.[21] After resolution of the discrepant results, the sensitivity and specificity rates, as well as positive and negative likely ratios for AMTDII were 88%, 99.2%, 110, and 0.11 for respiratory specimens and 74.3%, 100%, 740, and 0.26 for extrapulmonary specimens, respectively. The corresponding values for DBT were 94.5%, 99.6%, 235, and 0.05 for respiratory specimens and 92.3%, 100%, 920, and 0.07 for extrapulmonary specimens, respectively. Differences between both assays were associated with the presence of inhibitors.

A sensitive and specific NASBA assay was developed for the amplification and detection of M. pneumoniae 16S rRNA in respiratory specimens.[2] The sensitivity of this NASBA was comparable to that of a PCR targeted at the P1 adhesin gene. A commercially available NASBA NucliSens Basic Kit® assay[12] and a real-time NASBA assay for the detection of M. pneumoniae 16S rRNA in respiratory specimens were developed by the same group.[4] The intrarun variability coefficients for the real-time detection of specimens spiked with 50, 500, and 5000 color changing units (CCU) of M. pneumoniae were 26.4, 8.1, and 4.6 respectively; the interrun variation coefficients for the same inputs were 34.8, 10.7, and 13.9, respectively. The intrarun variation coefficients for the electrochemiluminescence detection of 50, 500, and 5000 CCU were 61.8, 45.3, and 28.2, respectively; the interrun variation coefficients for the same inputs were 57.7, 78.2, and 42.6 respectively. Loens et al. concluded that realtime NASBA, in comparison with ECL detection, shows high concordance in sensitivity and specificity with a clear advantage for the real-time technology regarding handling, speed, and number of samples that can easily be tested in a single run. Furthermore, the real-time NASBA assay requiring less manipulations and producing results without postamplification processing reduces the potential risk for product carryover.

Goessens et al.[22] compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the LCx assay (Abbott Laboratories, Chicago, IL), and the Roche COBAS AMPLICOR assay for the detection of C. trachomatis in urine samples. Sensitivities and specificities were calculated by using the following gold standard: true positive if two or more techniques yielded a positive result. The sensitivities of LCx, COBAS AMPLICOR, and AMP CT were 84%, 93%, and 85%, respectively. Specificity exceeded 99% for all three assays.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment