N

Signals:

Signals:

DQAl*01-04/06

DQAl*01-04/06

DQA1*01

DQAl*03-04/06

biotinylated Oligo DQAl*01a & DQAl*01b probes DQAl*02a & DQAl*02b probes DQA1*03 probe DQAl*04/06 probe DQA1*05 probe negative controls

Type:

DQAl*01-04/06

DQAl*01-04/06

DQA1*01

DQAl*03-04/06

Fig. 3 HLA-DQA1 typing of cervical carcinoma and control samples using DNA microarray: (Top) DNA microarray illustrating positions of probes for alleles 01, 02, 03, 04/06, and 05 in the HLA-DQA1 gene. On the right is probe nomenclature for the corresponding probe numbers on the DNA microarray. (Bottom) The same samples used for ''gold standard''were tested on the array. The spot diameter is 120-130 mm, with 500 mm spacing. Alleles found by DNA chip analysis are displayed by positive probe signals. All photographs were taken with the use of a CCD camera under a UV light source.

in 2 x SSC/0.1% SDS, add 50 mL of ELF substrate (Molecular Probes) to the slide and cover with a coverslip. Place in humidity chamber protected from light and incubate overnight. Results are obtained by viewing the slides with an ultraviolet (UV) transilluminator or with the use of a charged-couple device (CCD) camera over a UV light source.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment