Nist Reference Materials Program

It is essential to have high-throughput[1] and global scanning[2] methods that are low-cost and reliable clinical platforms to analyze large cohort studies, which statistically validate potential DNA, RNA, and protein assays. The National Institutes of Standards and Technology has developed expertise in measuring genomic instability of DNA repeat elements, the measurement of mutations in nuclear-encoded genes, and the measurement of DNA sequence variations in the mitochondrial genome. This is due, in part, to the development of a measurement program for human identification in conjunction with the Department of Justice.[3,4] In addition to examining current practices and assessing specific causes of measurement error, NIST has developed standard reference materials (SRMs) for optimizing the reproducibility of measurements for restriction fragment-length polymorphism (RFLP) and short tandem repeat (STR) alleles, and mitochondrial DNA sequences used by forensic community. In total, five SRMs are currently available for this purpose, with two additional materials underway.

For the NIST measurement programs, we followed the ACMG guidelines including the recommendation that validation studies incorporate well-characterized samples and positive and negative controls, thus providing evidence of generic and specific sequence amplification.1-5-1

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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