PCR Amplification

High-fidelity polymerase such as PFU (Stratagene) or Phusion (MJ research) should be used to reduce the possibility of PCR errors. In addition, PCR products should be analyzed by agarose gel electrophoresis prior to the transcription/translation. Direct analysis of the PCR products can indicate the presence of an abnormally sized amplicon suggesting a splicing error or genetic rearrangements such as deletions or duplications. However, purification of the PCR amplicon of the correct size is not necessary and should be avoided, because the aberrant-sized PCR products are often derived from mutant alleles.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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