Pmlrara Rtpcr

Mononuclear cells from the bone marrow or the peripheral blood were separated by dextran centrifugation, and cellular RNA was extracted with RNAsol according to the modified acid guanidium thiocyanate method, and reverse transcripted with avian myelomatous virus reverse transcriptase (Boehringer-Mannheim, Germany) with random primer according to the method of Borrow.[13]

The quantitative results of PML/RARA FISH and RARA FISH in 16 patients with acute myeloid leukemia (APL) at initial diagnosis showed good correlation (r=0.83): mean±S.D. was 95.3±6.5% for PML/RARA and 97.0±7.0% for RARA. The quantitative results of RARA FISH were rather higher than those of PML/RARA (Fig. 1). The quantitative results of PML/RARA FISH and RARA FISH in 21 patients with APL at follow-up were well correlated (r =0.75): mean±S.D. was 2.81 ±3.07%

Table 1 Analysis of the positive cases of RARA rearrangement FISH in comparison with results of PML/ RARA translocation FISH
Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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