Primer Design

Ideally, LPCR primers length should be 20 to 29 bases with no hairpins or 3' complementarity, and with Tm between 60°C and 68°C in 85 mM salt. Several software programs for primer design are available commercially, such as Primer Express (ABI Biosystems) and Oligo

(National Biosciences), among many others. To amplify as many orthopox viruses as possible, consensus or degenerate primers should be designed. Table 1 shows examples of consensus primers designed to amplify eight overlapping segments spanning about 98% of Orthopoxvirus genomes.

Was this article helpful?

0 0
Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment