Problem Low Amount of Defined PCR Product on High Background Smear

Reduce the number of cycles by steps of two. Reduce template DNA by decrements of 50 ng. Increase the time of the elongation step. Check the ratio of magnesium ions/dNTP. A surplus of free Mg2+ is required; therefore a final dNTP concentration at 500 mM requires 2.5 mM Mg2+. Reduce the amount of enzyme in decrements of 0.2 U. Increase the concentration of magnesium ions up to 3.5 mM in 0.25 increments if more than 500 ng of template DNA, or more than 500 mM dNTPs is used.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment