Protein Analysis

Proteins are the effector molecules of the cell and can be modified through a variety of posttranscriptional mechanisms. Therefore a cell-specific analysis of protein patterns may ultimately prove to be more informative than expression profiling in primary tissues. For these reasons, a variety of proteomic techniques have recently been applied to microdissected tissues, although the inherent limitation of starting material can present a major obstacle for protein analyses.[14-17] Nevertheless, standard techniques such as the two-dimensional polyacryl-amide electrophoresis for the detection of differential protein expression have been tested successfully on microdissected samples, and protocols for optimal fixation and protein recovery have been published.[18,19] Microdissection may also prove valuable to reduce sample complexity for mass spectrometric techniques such as surface-enhanced laser desorption/ionization time-of-flight (SELDI-ToF),[14] which need significantly less starting material than most gel-based techniques. Novel approaches such as the reverse-phase protein microarray[20] may prove useful to study multiple proteins, such as whole signal transduction pathways, in microdissected samples.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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