Protein Microarray Technology Technology Overview

One means of profiling a protein network via protein microarrays is accomplished by comparing the proportion of total protein (phosphorylated and nonphosphorylated) to the phosphorylated protein. Comparison of a selected set of protein phosphorylation events, coupled with knowledge of the biology of the phosphorylation event, may allow us to infer information concerning the activity level of the protein(s). Monitoring the total and phos-phorylated proteins before, during, or after treatment, or periodically over time, or between disease and disease-free states, may allow us to infer information concerning the activity levels of the proteins in real time for a

Sigrial network profile

Fig. 1 Protein molecular profiling. Patient biopsy samples are applicable for molecular profiling via laser capture microdissection (LCM), protein microarray, and bioinformatic analysis. A heterogeneous biopsy is the starting point for cell procurement. LCM enhances the procurement of pure cell populations. Whole-cell protein lysates are prepared from the microdissected cells and printed on a microarray. Bioinformatic analysis reveals the activation state of key signaling proteins. The pattern of activated or phosphorylated proteins is developed into a signaling network profile for that particular patient sample.

Sigrial network profile

Fig. 1 Protein molecular profiling. Patient biopsy samples are applicable for molecular profiling via laser capture microdissection (LCM), protein microarray, and bioinformatic analysis. A heterogeneous biopsy is the starting point for cell procurement. LCM enhances the procurement of pure cell populations. Whole-cell protein lysates are prepared from the microdissected cells and printed on a microarray. Bioinformatic analysis reveals the activation state of key signaling proteins. The pattern of activated or phosphorylated proteins is developed into a signaling network profile for that particular patient sample.

particular protein pathway.[14'15] It is conceivable that this technology concept could be applied to other posttrans-lational modification events such as glycosylation, lipidation, or myristalization.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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