Quantitation Of mRNA BY bDna Signal Amplification

bDNA technology during recent years has been increasingly adapted to the requirements of research laboratories and is now, for instance, a powerful tool for high-throughput mRNA quantitation from cell lysates or tissue homogenates. In this format, the assays are appropriate for a variety of research applications covering such different fields as regulation of gene expression, identification and validation of targets, secondary screening of biological agents, in vitro toxicology, metabolic and cell-signaling pathway studies, and cancer biology.[3,22] Although commercial kits for mRNA quantitation by bDNA signal amplification (QuantiGeneâ„¢) are available from Geno-spectra (Fremont, CA), users, of course, still have to design a target probe set for the gene of interest. In this respect, they may rely on the help of the already mentioned ProbeDesigner software package, which allows for the prediction of nonspecific hybridization events, thereby reducing the overall assay background.1-5-1

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Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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