Quantitation of Prognostic Markers in Neuroblastoma Tissue

A number of biochemical markers have been investigated on the gene level as to their relevance in relation to patient prognosis. Among these are N-MYC, NPY, and somatostatin 2 gene expression.[12] The first two will be discussed in detail.

known to correlate with the differentiation and malignancy of neuroblastomas. NPY is released into the circulation and may reflect the tumor burden of an individual neu-roblastoma patient.

Consequently, NPY gene expression has been quantified by using a number of different techniques for quantitation such as Northern blot, competitive RT-PCR, and, lately, TaqMan real-time PCR.[11]

There is a good linear relationship between NPY mRNA expression as measured by quantitative competitive PCR and TaqMan real-time PCR. However, in the lower concentration range, only real-time PCR is able to discriminate different NPY mRNA levels. This finding suggests that the more traditional competitive PCR might not be able to detect differences in the expression of less abundant genes in neuroblastoma tissues. Definitely, with a limited amount of cells, real-time PCR would be the more desirable method to use.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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