Quantitative RTPCR

Real-time RT-PCR affords sensitive, rapid, and reproducible quantification of mRNA/cDNA transcripts. Although sensitivities are somewhat less than with conventional or nested RT-PCR, the dynamic range of fluorescent detection is much broader, spanning five to six orders of magnitude, with a lower limit of detection of <0.01% (10"4).[12] Most importantly, real-time RT-PCR permits the serial precision required in clinical diagnostic applications.

Various fluorescent detection systems have been used to quantify BCR-ABL. The major chemistries employed are the use of Taqman™ or FRET probes. For both of these, RNA or cDNA standards of known concentration

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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