Rapid LAMP Method

To accelerate the LAMP reaction, loop primers act from the elongation steps of the original LAMP reaction (Fig. 2, lower). The loop primer called loop B hybridizes to single-stranded loop region of the stem-loop DNA in structure 9 before initiating strand displacement DNA synthesis. Then, DNA synthesized from this loop primer is displaced by extension from the 3' end of the target DNA, generating structure 11, which is not created in the original LAMP reaction. Structures 6 and 8 generated by loop primers are also obtained in the original method and are reused in the LAMP reaction. All of the stem-loop DNAs are used by either the inner or loop primers, e.g., structures 14, 15, and 16. The loop primers hybridize to the stem-loop region in structure 9, as well as in the other structures that are generated by the LAMP reaction, resulting in dramatic amplification.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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