Rapidity and High Sensitivity

Because the LAMP method has a high specificity, the target gene alone can be amplified with high efficiency. In

Time (min)

Fig. 1 Detection of LAMP products. (A) Electrophoresis of the LAMP products. Various copy numbers of HBV DNA were amplified at 60°C for 60 min. (B) Real-time detection using turbidity. The LAMP reaction was conducted with 6000 copies DNA at 65°C. The inset shows the tube after reaction. (View this art in color at www.dekker.com.)

Time (min)

Fig. 1 Detection of LAMP products. (A) Electrophoresis of the LAMP products. Various copy numbers of HBV DNA were amplified at 60°C for 60 min. (B) Real-time detection using turbidity. The LAMP reaction was conducted with 6000 copies DNA at 65°C. The inset shows the tube after reaction. (View this art in color at www.dekker.com.)

fact, it is possible to detect a target gene within 1hr. We have also developed a method of rapid gene amplification by adding new primers called loop primers to amplify the loop region of products with which the inner primers do not anneal.[3] The LAMP method, like PCR, has a sensitivity of a few copies (Fig. 1A). Because a strand substitution reaction is used for the synthesis, no amplicon leads to product inhibition during the amplification, so that the yield of the products reaches as high as 0.5 mg/mL.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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