Rca Basics

RCA is based on the rolling replication of short, single-stranded DNA circles by certain DNA polymerases at constant temperature, the process discovered in the mid-1990s.[1,2] This reaction is initiated by the hybridization of a linear DNA single strand to a specific DNA mini-circle, and it is widely used for diagnostic purposes in direct or indirect detection of different DNA/RNA, protein, and other biomarkers via a set of various biomolecular recognition events. A similar reaction was described for RNA polymerases as well,[3] but the RNA-generated process does not require any hybridization-dependent priming (or even promoter sequences). Therefore, the latter is only used to produce functional RNA sequences, such as RNA ladders and self-processing ribozymes.

Description of Method

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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