References

1. Livak, K.J.; Flood, S.J.A.; Marmaro, J.; Mullah, K.B. Hybridization Assay Using Self-Quenching Fluorescence Probe. US Patent 5,876,930, March, 1999. Applied Biosystems: Foster City, CA.

2. Bernard, P.S.; Lay, M.J.; Wittwer, C.T. Integrated amplification and detection of the C677T point muta tion in the methylenetetrahydrofolate reductase gene by fluorescence resonance energy transfer and probe melting curves. Anal. Biochem. 1998, 255 (1), 101107.

3. Tyagi, S.; Kramer, F.R. Molecular beacons: Probes that fluoresce upon hybridization. Nat. Biotechnol. 1996, 14 (3), 303-308.

4. Walburger, D.K.; Afonina, I.A.; Wydro, R. An improved real time PCR method for simultaneous detection of C282Y and H63D mutations in the HFE gene associated with hereditary hemochromatosis. Mutat. Res. 2001, 432 (3-4), 69-78.

5. Wittwer, C.T.; Herrmann, M.G.; Moss, A.A.; Rasmussen, R.P. Continuous fluorescence monitoring of rapid cycle DNA amplification. BioTechniques 1997, 22 (1), 130-131, 134-138.

6. Ririe, K.M.; Rasmussen, R.P.; Wittwer, C.T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 1997, 245 (2), 154-160.

7. Vet, J.A.; Majithia, A.R.; Marras, S.A.; Tyagi, S.; Dube, S.; Poiesz, B.J.; Kramer, F.R. Multiplex detection of four pathogenic retroviruses using molecular beacons. Proc. Natl. Acad. Sci. U. S. A. 1999, 96 (11), 63946399.

8. Bernard, P.S.; Ajioka, R.S.; Kushner, J.P.; Wittwer, C.T. Homogeneous multiplex genotyping of hemochromatosis mutations with fluorescent hybridization probes. Am. J. Pathol. 1998, 153 (4), 1055-1061.

9. Exner, M.M.; Lewinski, M.A. Sensitivity of multiplex realtime PCR reactions, using the LightCycler and the ABI PRISM 7700 Sequence Detection System, is dependent on the concentration of the DNA polymerase. Mol. Cell. Probes 2002, 16 (5), 351-357.

10. Corless, C.E.; Guiver, M.; Borrow, R.; Edwards-Jones, V.; Fox, A.J.; Kaczmarski, E.B. Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR. J. Clin. Microbiol. 2001, 39 (4), 1553-1558.

11. Ugozzoli, L.A.; Chinn, D.; Hamby, K. Fluorescent multicolor multiplex homogeneous assay for the simultaneous analysis of the two most common hemochro-matosis mutations. Anal. Biochem. 2002, 307 (1), 4753.

12. Sanchez, J.L.; Storch, G.A. Multiplex, quantitative, realtime PCR assay for cytomegalovirus and human DNA. J. Clin. Microbiol. 2002, 40 (7), 2381-2386.

13. Bijwaard, K.E.; Fetsch, J.F.; Przygodzki, R.; Taubenberger, J.K.; Lichy, J.H. Detection of SYT-SSX fusion transcripts in archival synovial sarcomas by real-time reverse transcriptase-polymerase chain reaction. J. Mol. Diagnostics 2002, 4 (1), 59-64.

14. Sloan, L.M.; Hopkins, M.K.; Mitchell, P.S.; Vetter, E.A.; Rosenblatt, J.E.; Harmsen, W.S.; Cockerill, F.R.; Patel, R. Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens. J. Clin. Microbiol. 2002, 40 (1), 96-100.

15. Layfield, L.J.; Bernard, P.S.; Goldstein, N.S. Color multi- 18. plex polymerase chain reaction for quantitative analysis of epidermal growth factor receptor genes in colorectal adenocarcinoma. J. Surg. Oncol. 2003, 83 (4), 227231. 19.

16. Von Ahsen, N.; Oellerich, M.; Schutz, E. A method for homogeneous color-compensated genotyping of factor V (G1691A) and methylenetetrahydrofolate reductase (C677T) mutations using real-time multiplex fluorescence PCR. Clin. Biochem. 2000, 33 (7), 535-539.

17. Templeton, K.E.; Scheltinga, S.A.; Sillekens, P.; 20. Crielaard, J.W.; van Dam, A.P.; Goossens, H.; Claas,

E.C. Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other

Legionella species. J. Clin. Microbiol. 2003, 41 (9), 4016-4021.

O'Neill, H.J.; Wyatt, D.E.; Coyle, P.V.; McCaughey, C.; Mitchell, F. Real-time nested multiplex PCR for the detection of Herpes simplex virus types 1 and 2 and Varicella zoster virus. J. Med. Virol. 2003, 71 (4), 557-560. Siraj, A.K.; Ozbek, U.; Sazawal, S.; Sirma, S.; Timson, G.; Al-Nasser, A.; Bhargava, M.; El Solh, H.; Bhatia, K.; Gutierrez, M.I. Preclinical validation of a monochrome real-time multiplex assay for translocations in childhood acute lymphoblastic leukemia. Clin. Cancer Res. 2002, 8 (12), 3832-3840.

De Kok, J.B.; Wiegerinck, E.T.; Giesendorf, B.A.; Swinkels, D.W. Rapid genotyping of single nucleotide polymorphisms using novel minor groove binding DNA oligonucleotides (MGB probes). Human Mutat. 2002, 19 (5), 554-559.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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