1. Niesters, H.G. Molecular and diagnostic clinical virology in real time. Clin. Microbiol. Infect. 2004, 10, 5-11.

2. Germer, S.; Holland, M.J.; Higuchi, R. High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR. Genome Res. 2000,10, 258-266.

3. Hazbon, M.H.; Alland, D. Hairpin primers for simplified single-nucleotide polymorphism analysis of Mycobacteri-um tuberculosis and other organisms. J. Clin. Microbiol. 2004, 42, 1236-1242.

4. Elenitoba-Johnson, K.S.; Bohling, S.D.; Wittwer, C.T.; King, T.C. Multiplex PCR by multicolor fluorimetry and fluorescence melting curve analysis. Nat. Med. 2001, 7, 249-253.

5. Wittwer, C.T.; Reed, G.H.; Gundry, C.N.; Vandersteen, J.G.; Pryor, R.J. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin. Chem. 2003, 49, 853-860.

6. Hernandez, M.; Rodriguez-Lazaro, D.; Esteve, T.; Prat, S.; Pla, M. Development of melting temperature-based SYBR Green I polymerase chain reaction methods for multiplex genetically modified organism detection. Anal. Biochem. 2003, 323, 164-170.

7. Gundry, C.N.; Vandersteen, J.G.; Reed, G.H.; Pryor, R.J.; Chen, J.; Wittwer, C.T. Amplicon melting analysis with labeled primers: A closed-tube method for differentiating homozygotes and heterozygotes. Clin. Chem. 2003, 49, 396-406.

8. Nazarenko, I.A.; Bhatnagar, S.K.; Hohman, R.J. A closed tube format for amplification and detection of DNA based on energy transfer. Nucleic Acids Res. 1997, 25, 25162521.

9. Nazarenko, I.; Lowe, B.; Darfler, M.; Ikonomi, P.; Schuster, D.; Rashtchian, A. Multiplex quantitative PCR using self-quenched primers labeled with a single fluo-rophore. Nucleic Acids Res. 2002, 30, e37.

10. Abbaszadegan, M.R.; Struewing, J.P.; Brown, K.M.; Snider, J.V.; Goodsaid, F.; Gore-Langton, R.; Hughes, M.R. Automated detection of prevalent mutations in BRCA1 and BRCA2 genes, using a fluorogenic PCR allelic discrimination assay. Genet. Test. 1997, 1, 171 -180.

11. Aslanidis, C.; Nauck, M.; Schmitz, G. High-speed prothrombin G ! A 20210 and methylenetetrahydrofolate 21. reductase C! T 677 mutation detection using real-time fluorescence PCR and melting curves. BioTechniques 1999, 27, 234-238. 22.

12. Mhlanga, M.M.; Malmberg, L. Using molecular beacons to detect single-nucleotide polymorphisms with real-time PCR. Methods 2001, 25, 463-471.

13. Tapp, I.; Malmberg, L.; Rennel, E.; Wik, M.; Syvanen, A.C. 23. Homogeneous scoring of single-nucleotide polymorphisms: Comparison of the 5'-nuclease TaqMan assay and molecular 24. beacon probes. BioTechniques 2000, 28, 732-738.

14. Tyagi, S.; Marras, S.A.; Kramer, F.R. Wavelength-shifting 25. molecular beacons. Nat. Med. 2000, 18, 1191-1196.

15. Solinas, A.; Brown, L.J.; McKeen, C.; Mellor, J.M.; Nicol, J.; Thelwell, N.; Brown, T. Duplex Scorpion primers in

SNP analysis and FRET applications. Nucleic Acids Res. 26. 2001, 29, E96.

16. French, D.J.; Archard, C.L.; Andersen, M.T.; McDowell, D.G. Ultra-rapid DNA analysis using HyBeacon probes and direct PCR amplification from saliva. Mol. Cell. 27. Probes 2002, 16, 319-326.

17. Timcheva, I.I.; Maximova, V.A.; Deligeorgiev, T.G.; Gadjev, N.I.; Sabnis, R.W.; Ivanov, I.G. Fluorescence 28. spectral characteristics of novel asymmetric monomethine cyanine dyes in nucleic acid solutions. FEBS Lett. 1997,

18. Afonina, I.A.; Reed, M.W.; Lusby, E.; Shishkina, I.G.; Belousov, Y.S. Minor groove binder-conjugated DNA 29. probes for quantitative DNA detection by hybridization-triggered fluorescence. BioTechniques 2002, 32, 940-949.

19. Bustin, S.A.; Nolan, T. Chemistries. In A-Z of Quantitative PCR; Bustin, S.A., Ed.; URL Press: La Jolla, CA, 2004.

20. Sanchez, J.A.; Pierce, K.E.; Rice, J.E.; Wangh, L.J. Linear- 30. after-the-exponential (LATE)-PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis. Proc. Natl. Acad. Sci. U. S. A. 2004, 101, 1933- 31. 1938.

Fiandaca, M.J.; Hyldig-Nielsen, J.J.; Gildea, B.D.; Coull, J.M. Self-reporting PNA/DNA primers for PCR analysis. Genome Res. 2001, 11, 609-613.

Johnson, M.P.; Haupt, L.M.; Griffiths, L.R. Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR. Nucleic Acids Res. 2004, 32, e55. Mackay, I.M. Real-time PCR in the microbiology laboratory. Clin. Microbiol. Infect. 2004, 10, 190-212. Mackay, I.M.; Arden, K.E.; Nitsche, A. Real-time PCR in virology. Nucleic Acids Res. 2002, 30, 1292-1305. Probert, W.S.; Bystrom, S.L.; Khashe, S.; Schrader, K.N.; Wong, J.D. 5' Exonuclease assay for detection of serogroup Y Neisseria meningitidis. J. Clin. Microbiol. 2002, 40, 4325-4328.

Esaki, H.; Noda, K.; Otsuki, N.; Kojima, A.; Asai, T.; Tamura, Y.; Takahashi, T. Rapid detection of quinolone-resistant Salmonella by real time SNP genotyping. J. Microbiol. Methods 2004, 58, 131-134. McKillip, J.L.; Drake, M. Real-time nucleic acid-based detection methods for pathogenic bacteria in food. J. Food Prot. 2004, 67, 823-832.

Qi, Y.; Patra, G.; Liang, X.; Williams, L.E.; Rose, S.; Redkar, R.J.; DelVecchio, V.G. Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis. Appl. Environ. Microbiol.

2001, 67, 3720-3727.

Vaitilingom, M.; Pijnenburg, H.; Gendre, F.; Brignon, P. Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods. J. Agric. Food Chem. 1999, 47, 5261-5266.

Ginzinger, D.G. Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream. Exp. Hematol. 2002, 30, 503-512. Walker, N.J. A technique whose time has come. Science

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment