References

1. Lyamichev, V.; Brow, M.A.; Varvel, V.E.; Dahlberg, J.E. Comparison of the 5' nuclease activities of Taq DNA polymerase and its isolated nuclease domain. Proc. Natl. Acad. Sci. U. S. A. 1999, 96, 6143-6148.

2. Lie, Y.S.; Petropoulos, C.J. Advances in quantitative PCR technology: 5' Nuclease assays. Curr. Opin. Biotechnol. 1998, 9, 43-48.

3. Mackay, I.M.; Arden, K.E.; Nitsche, A. Real-time PCR in virology. Nucleic Acids Res. 2002, 30, 12921305.

4. De Angelis, D.A. Why FRET over genomics? Physiol. Genomics 1999, 1, 93-99.

5. Lyamichev, V.; Brow, M.A.; Dahlberg, J.E. Structure-specific endonucleolytic cleavage of nucleic acids by eu-bacterial DNA polymerases. Science 1993, 260, 778783.

6. Holland, P.M.; Abramson, R.D.; Watson, R.; Gelfand, D.H. Detection of specific polymerase chain reaction product by utilizing the 5'-3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. U. S. A. 1991, 88, 7276-7280.

7. Lee, L.G.; Connell, C.R.; Bloch, W. Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Res. 1993, 21, 3761-3766.

8. Livak, K.J.; Flood, S.J.; Marmaro, J.; Giusti, W.; Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. 1995, 4, 357-362.

9. Heid, C.A.; Stevens, J.; Livak, K.J.; Williams, P.M. Real time quantitative PCR. Genome Res. 1996, 6, 986-994.

10. Gibson, U.E.; Heid, C.A.; Williams, P.M. A novel method for real time quantitative RT-PCR. Genome Res. 1996, 6, 995-1001.

11. Kreuzer, K.A.; Bohn, A.; Lupberger, J.; Solassol, J.; le Coutre, P.; Schmidt, C.A. Simultaneous absolute quantification of target and control templates by real-time fluorescence reverse transcription-PCR using 4-(4'-dime-thylaminophenylazo)benzoic acid as a dark quencher dye. Clin. Chem. 2001, 47, 486-490.

12. Kutyavin, I.V.; Afonina, I.A.; Mills, A.; Gorn, V.V.; Lukhtanov, E.A.; Belousov, E.S.; Singer, M.J.; Walburger, D.K.; Lokhov, S.G.; Gall, A.A.; Dempcy, R.; Reed, M.W.; Meyer, R.B.; Hedgpeth, J. 3'-minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Res. 2000, 28, 655-661.

Was this article helpful?

0 0
Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment