Relative Quantification

Relative quantification expresses the changes in steady-state mRNA levels of a gene relative to the levels of a coamplified internal control mRNA.[25] Target Ct values are compared directly to an internal reference Ct and results are expressed as ratios of the target-specific signal to the internal reference. This produces a corrected relative value for the target-specific mRNA product, which can be compared between samples and allows an estimate of the relative expression of target mRNA in those samples. Amplification efficiencies of a target and a reference must be similar because they directly affect the accuracy of any calculated expression result and must be incorporated into copy number calculations.1-26,27-1 However, because the expression of the internal control itself is often variable, relative quantification can be misleading.1-28-1

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment