Reliability And Specificity Of Realtime

The use of TaqMan real-time PCR for the quantification of gene expression in neuroblastoma tissues has been shown to be at least as reliable as the application of other quantitative PCR techniques such as competitive PCR.[11] Whereas the expression of highly expressed genes, such as the housekeeping gene glyceraldehyde-3-phosphate de-hydrogenase, is well correlated between the two methods for the determination of genes with lower expression such as neuropeptide Y (NPY), TaqMan PCR is much more sensitive than competitive PCR in neuroblastoma tissues.[11] In addition, the spectrum of linear measurements for real-time PCR is in the range of 106, in contrast to 102 in competitive RT-PCR. Finally, a considerably higher number of samples per day can be measured by real-time PCR (up to 400 measurements). In comparison to the Northern blot assessment, only a minimal fraction of mRNA is necessary to quantify gene expression by real-

time PCR.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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