Robustness And Specificity Of Galios Genotyping

Upon variation of the amount of genomic DNA from 100 to 1 ng per 20 pL reaction, unambiguous genotype discrimination was obtained (Fig. 3A), illustrating the robustness of GALIOSTM against variations in template concentration.

The specificity of GALIOSTM genotyping was examined by parallel analysis of 27 gDNAs (10, 10, and 7 for hMLH1 genotypes A/A, A/G, and G/G, respectively) with and without addition of 10 ng yeast gDNA representing a 500-fold molar excess of ''contaminating'' template. The results of the FCS analysis show that accuracy and precision of genotyping results are not affected by the presence of excess unspecific DNA (Fig. 3B), thus proving the very high specificity of the system.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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