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Fig. 3 Robustness, specificity, and miniaturization of GALIOSTM genotyping. (A) Plot of the NFS values obtained for hMLHl A655G genotypes (A/A, A/G, and G/G) using 100-1 ng of gDNA per reaction. (B) Box plot illustrating the highly precise distribution of NFS values obtained for each hMLH1 A655G genotype from several (n = 7-10) gDNAs both in the absence (—yeast DNA) or presence (+ yeast DNA) of unspecific template. Median, S.D., and CI 99.99% values are denoted at the respective boxes. (C) Plot of the NFS values obtained for each hMLH1 A655G genotype obtained from standard and miniaturized reaction volumes (50-1 pL) containing a gDNA concentration of 1 ng/pL. (View this art in color at www.dekker.com.)

Fig. 3 Robustness, specificity, and miniaturization of GALIOSTM genotyping. (A) Plot of the NFS values obtained for hMLHl A655G genotypes (A/A, A/G, and G/G) using 100-1 ng of gDNA per reaction. (B) Box plot illustrating the highly precise distribution of NFS values obtained for each hMLH1 A655G genotype from several (n = 7-10) gDNAs both in the absence (—yeast DNA) or presence (+ yeast DNA) of unspecific template. Median, S.D., and CI 99.99% values are denoted at the respective boxes. (C) Plot of the NFS values obtained for each hMLH1 A655G genotype obtained from standard and miniaturized reaction volumes (50-1 pL) containing a gDNA concentration of 1 ng/pL. (View this art in color at www.dekker.com.)

543 and 633 nm. Mean amplimer values from triplicate measurements were used to calculate 10 individual NFSs per genotype. For intersample precision, 10, 10, and 7 individual gDNAs for hMLHl genotypes A/A, A/G, and G/G, respectively, were amplified and measured by FCS for 3 x 3 sec each at both 543 and 633 nm. Mean amplimer values from triplicate measurements were used to calculate individual NFSs.

All precision experiments yielded fully reproducible results and the NFS values showed a narrow distribution for each hMLHl genotype (Fig. 2E), thus indicating the high reproducibility and precision of GALIOS™ genotyping.

Accuracy, reproducibility, and precision of GALIOSTM genotyping were highly identical when FCS measurement time was reduced to 1 x 2 sec at both 543 and 633 nm (data not shown).

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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