Many different techniques such as single-strand conformation polymorphism (SSCP),[7] hydroxilamine and osmium tetroxide (HOT) chemical cleavage,[8] protein truncation test (PTT),[9] and heteroduplex analysis (HA)[4,5] are available to identify single nucleotide variants. However, in many cases, there is not a very high mutation detection rate when we use each one of

Denaturing Gradient Gel Electrophoresis (DGGE) Mutation Control

Denaturing Gradient Gel Electrophoresis (DGGE) Mutation Control

Fig. 1 Small mutation detection in exon 17 of the dystrophin gene.

these techniques principally because of technical difficulties. For example, RNA-based techniques are difficult to use in a diagnostic setting because RNA expression is oftentimes extremely low.

Some laboratories are using haplotype analysis to assess risk status; however, this strategy may be inconclusive because in many cases there is intragenic recombination and high mutation rate. This represents a great obstacle to genetic analysis of patients with small mutations and genetic counseling of their relatives.

Recently, many diagnostic and research laboratories are using denaturing high-performance liquid chromatog-raphy (DHPLC)[10] to scan for single nucleotide variations, but even with this method (best detection rate close to 92%) many families remain excluded from the analysis because the detection rate is not 100%. It is important to know that only mutation detection gives absolute certainty about diagnostics and counseling.

Denaturing gradient gel electrophoresis is the most sensitive electrophoretic method (mutation detection rate close to 100%)[1] and, despite this, does not require radioisotope use, is less labor intensive and time consuming, is easy to reproduce, and is usually cheaper than the other routinely used methods for point mutation detection.[7]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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