The detection sensitivities of colony PCR for E. coli, Aeromonas hydrophila, Campylobacter jejuni, and S. aureus were determined by both PCR and plate count methods using agarose gel electrophoresis relative to actual plate counts within the range of 10 to 1013 colony forming units per milliliter (CFU/mL). The detection ranges of colony PCR (in CFU/mL) for E. coli, A. hydrophila, C. jejuni, and S. aureus were 8 x 105-10, 3 x 104-11, 1 x 104-13, and 3 x 108-12, respectively. However, the capability of detecting the target gene can be reduced by the presence of higher concentrations of nontarget DNA or PCR inhibitors.[10,11] It appears that a range of 109 to 1010 CFU/mL was most effective for all of the colony PCR assays and only about 10 microorganisms were required for the detection of Mycobacterium tuberculosis.[6,8]

Was this article helpful?

0 0
Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment