Study Design and Desired Typing Resolution

For mucosal HPVs, a simple detection of HR-HPV without typing may be sufficient in many study designs. These include population-based screening studies for cervical cancer, in particular, the identification of women with abnormal cervical cytology who are at risk of progressive cervical intraepithelial neoplasia (CIN) disease[19] and the follow-up after treatment of CIN.[20] The method of choice in such studies would be hybridization with a HR probe cocktail, either after consensus PCR or using HC2.

Other studies may call for high-resolution HPV typing. These include studies of possible relations between HPV type and clinicopathological aspects such as histology (e.g., mucosal HPVs in adenocarcinomas, SCCs, and small-cell carcinomas of the uterine cervix; cutaneous HPVs in different cutaneous diseases) and patient survival. Furthermore, HPV typing can be helpful to determine whether two HPV-associated lesions within the same patient are pathogenetically related (e.g., a cervical carcinoma and an earlier detected CIN lesion).

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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