The reference panel for the detection of TP53 mutations was created from site-directed mutagenesis and cloned into a renewable plasmid vector. Validation of the panel was performed by direct DNA sequencing and with common mutation scanning technologies.
Our results demonstrate that these reference materials and methods can be used in the clinical diagnosis of TP53 mutations in heritable and somatic disease. They may also be useful in the validation of other mutation detection technologies. The panel provides internal quality control and lowers the inherent error of measurements by ensuring that protocol optimization can always detect the mutations contained in the panel. Hence each diagnostic laboratory can adjust its ''in-house'' protocols to obtain expected and normalized results that can be readily shared and easily compared between different diagnostic laboratories using different analytical techniques.
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.