Target for Detection

The detection target is mainly a part of the pX gene of the HTLV-1 provirus integrated within the host genome, which is highly conserved even in defective viruses.

However, despite a high degree of homology among the HTLV-1 strains, deletions or mutations in the pX region have been described especially in patients with HAM/ TSP. As specimens for quantifying the proviral load, fresh or frozen peripheral blood mononuclear cells (PBMCs) are used most often. After being collected by Ficoll-Conray gradient centrifugation, the genomic DNA is processed by the phenol-chloroform method. The ge-nomic DNA is stored at 4°C until use. Genomic DNA doses applied to PCR assay should be precisely quantified using the dsDNA PicoGreen Quantification Kit (Molecular Probes, Eugene, OR, USA) to make precise results.

Was this article helpful?

0 0
Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment