The detection target is mainly a part of the pX gene of the HTLV-1 provirus integrated within the host genome, which is highly conserved even in defective viruses.
However, despite a high degree of homology among the HTLV-1 strains, deletions or mutations in the pX region have been described especially in patients with HAM/ TSP. As specimens for quantifying the proviral load, fresh or frozen peripheral blood mononuclear cells (PBMCs) are used most often. After being collected by Ficoll-Conray gradient centrifugation, the genomic DNA is processed by the phenol-chloroform method. The ge-nomic DNA is stored at 4°C until use. Genomic DNA doses applied to PCR assay should be precisely quantified using the dsDNA PicoGreen Quantification Kit (Molecular Probes, Eugene, OR, USA) to make precise results.
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.