Tat Aaa Gtc Cat Tgc Ac

Type specific

Where Y=thymine or cytosine.

Where Y=thymine or cytosine.

Negative stain electron microscopy was the method first used to detect rotavirus. Its major advantages are that virus is detected by its distinctive morphology (Fig. 1), so the test is highly specific and the technology is ''catch-all'' in that other viral enteropathogens can also be detected. Its disadvantages are that it requires specialized expensive equipment with an experienced electron microscopist and that to be detectable there must be over 1 million viral particles per milliliter of sample.

A number of antigen detection formats have been designed including latex particle agglutination (LPA) and enzyme-linked immunosorbent assays (ELISA). In general, ELISA is more sensitive than LPA but with both there are problems of specificity. Latex particle agglutination has the advantage over ELISA of being in a format for single tests whereas ELISA is better for batch testing. Both are available as commercial kits.

Genome detection is either by PAGE of dsRNA extracted from stool or RT-PCR. Silver-stained PAGE gels are of equivalent sensitivity and specificity to electron microscopy. However, RT-PCR is the most sensitive technique but there are no commercial kits available. Using RT-PCR has the added benefit of allowing virus genotyping. Some of the PCR oligonucleotide primers available for detection and genotyping are shown in Tables 5 and 6.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment