Technical Description

Southern blotting has been used for many years to detect and locate genomic sequences from a variety of pro-karyotic and eukaryotic organisms. For gene detection, whole chromosomal DNA is digested with a restriction enzyme, and the fragments are separated by electropho-resis through an agarose gel. The separated fragments are transferred from the agarose gel to either a nitrocellulose or a nylon membrane by Southern blotting (Fig. 1). The membrane-bound nucleic acid is then hybridized to one or more labeled probes homologous to the gene to be examined. Probes can be labeled with a number of detectable moieties, including colorimetric and chemilu-minescent enzymes to bus with the appropriate substrates. This classical method has been adapted to differentiate bacterial strains on the basis of the observation that the location of various restriction enzyme recognition sites within a particular genetic locus of interest can be polymorphic from strain to strain, resulting in gel


Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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