Technical Description The Competitor Templates

Competitive PCR can be adopted for both DNA and mRNA measurements and generally requires the construction of synthetic DNA or RNA competitors.[1'2] DNA competitors have the advantage of better stability and more accurate quantification methods in comparison to RNA competitive templates. Whereas, if DNA is used, competitor quality and quantity can be compared with stable DNA standard preparations, the measurement of mRNA is complicated by the difficulty of obtaining a practicable reference standard for the calibration of the RNA competitor. The use of a DNA competitor for mRNA measurements may simplify the procedure of quantitative reverse transcript PCR (RT-PCR). However, the use of a DNA standard for mRNA measurements, even if it represents an advantage because of its stability and ease of handling,1-3-1 cannot prevent the inaccuracy of RT-

PCR which is due to the large variability of reverse transcription efficiency.

In some cases, a DNA competitor can be obtained by selecting the primers on two contiguous exons separated by a small intron, but generally, the competitor must be synthesized ad hoc. Competitors are normally cloned in plasmid vectors, transcribed in vitro to obtain large amounts of specific transcript, and measured precisely to determine competitor concentration.1-4-1

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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