The basic theory behind inverse PCR requires a core DNA region of known sequence to be adjacent to an unknown target. This region is digested to produce a fragment of suitable size for PCR that is ligated together to produce a circular construct. The core region provides the sequence information required for synthesis of primers, designed to hybridize to the ends of this core region, which, because of the nature of the circular construct, flank the unknown sequence. Extension occurs divergently toward the unknown sequence with PCR amplification of the uncharacterized region.
Was this article helpful?
The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.