Technical Overview

The principle of LD-PCR is essentially the same as for any standard PCR technique, with primers specific for two known regions flanking a target DNA sequence, enabling quick and efficient exponential amplification of the target amplicon. Standard PCR procedures tend to use only Taq polymerase, whereas in LD-PCR a proofreading enzyme (Pfu polymerase; from Pyrococcus fuirosus) must be used to correct possible nucleotide mismatches and prevent stalling of the extension process or introduction of a mutation into the sequence. Furthermore, it is preferential to replace the Taq polymerase with a more thermostable enzyme such as rTth DNA polymerase (the recombinant form of the DNA polymerase from Thermus thermophi-lus). As with all PCR procedures, the reaction conditions and reagents must be optimized, but for LD-PCR it is also beneficial to obtain a high-quality (purity and length) DNA template, design primers optimally, and use a buffer composition optimal for the polymerase activity.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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