Template Considerations for PCR Amplification

Although transcription/translation of PCR products up to 3 kb is not difficult, best results are obtained using fragments of 1.3-2.0 kb size. If a gene contains several kilobases of coding sequence, it is necessary to divide it into multiple segments. Most published PTT reports use genomic DNA as the template to analyze large exons, e.g., exon 11 of BRCA1 and exon 15 of the APC genes.[1,3]

Cells or Tissue or Blood Sample t

DNA/mRNA isolation

Disadvantages

Conventional PTT

Target Gene Amplification by PCR/RT-PCR

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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