The Capillary

Capillaries can be made of fused silica, glass, or Teflon. The most popular is fused silica, which is externally coated with polyimide to give it greater strength and flexibility. The polyimide layer is usually removed at the detector end to enable online detection. Capillary lengths range from 10 to 100 cm, and internal diameters vary from 20 to 100 mm. These narrow bores improve heat dissipation due to a higher surface area to volume ratio.

Manufacturers recommend that capillaries are changed after a defined number of injections in diagnostic work. In our laboratory, the capillaries are replaced after 200 injections. Multichannel CGE, such as that used in the ABI Prism 3700 DNA Analyzer®, allows simultaneous

Fig. 1 Simple representation of a CGE apparatus. The + and — represent the anode and cathode, respectively, joined through a capillary (solid line). The sample is loaded automatically and the end products are detected through a laser-CCD system (IS). Software allows the injection and electrophoresis conditions to be selected, and fragments to be characterized based on their size and color. (View this art in color at www.dekker.com.)

Fig. 1 Simple representation of a CGE apparatus. The + and — represent the anode and cathode, respectively, joined through a capillary (solid line). The sample is loaded automatically and the end products are detected through a laser-CCD system (IS). Software allows the injection and electrophoresis conditions to be selected, and fragments to be characterized based on their size and color. (View this art in color at www.dekker.com.)

analysis of information from 96 capillaries, thereby facilitating high-throughput DNA analysis.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment