The Prion Protein

The primary translation product of PRNP contains signal peptides of 22 or 23 amino acids at the N- and C-termini, respectively, which are both removed during intracellular processing of the nascent PrP. The mature PrP 23-230 is membrane-anchored through a C-terminal glycosyl-phosphatidyl-inositol (GPI) anchor unit, and two aspar-agine glycosylation sites within the protein can give rise to three glycotypes (unglycosylated, monoglycosylated, and diglycosylated). Structurally, the protein features a fivefold repeat of eight amino acids each (octa-repeats) toward the N-terminus, and a globular domain that contains a short, two-stranded antiparallel beta sheet and three alpha helices in the C-terminal half.[9]

According to the protein-only hypothesis, the hallmark of the infectious process for acquired (iatrogenic or foodborne), sporadic, and inherited prion diseases is recruitment of the cellular PrP to form a self-replicating conformational PrP isomer, ultimately accumulating into aggregated deposits. As soon as this conversion is initiated, different prion strains replicate faithfully upon transmission into new hosts.

Because most available anti-PrP reagents recognize the normal and the abnormal isoform of the PrP, digestion with proteinase K is experimentally used to completely degrade the normal isoform. The same treatment results in a protease-resistant core with an apparent molecular mass of 27-30 kDa (PrP 27-30) for the pathological isoform.[10]

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Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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