Troubleshooting Problem No PCR Product

Check the quality of primers and template.

Increase the number of cycles (by steps of five).

Increase template DNA by 50-ng increments.

Increase enzyme concentration (up to 5 U/50 mL).

Check if the PCR tubes fit the thermocycler used. Poor thermal contact prevents effective temperature transfer.

Check for air bubbles trapped after mixing the PCR components. Air bubbles prevent homogenous temperature distribution throughout the reaction volume.

Heat denaturation: 95°C 1 Annealing: 50°C

Heat denaturation: 95°C 1 Annealing: 50°C

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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