Tta Gtg Agc Cac Cag Cca Tc Ggt Gtc Aca Gga Cca Aaa Cc

detect each of the eight astrovirus serotypes (IDEIA Astrovirus, Dako Diagnostics, United Kingdom), and this has been shown to have a sensitivity of 100% and specificity of 98.6%.[31]

Detection of virus genome by RT-PCR is estimated to detect as few as 10 to 100 astrovirus particles.[32] A number of different targets have been used. One strategy employs primers (Mon244 and Mon245) which amplify a 413-nt fragment of ORF2 (Table 1), and if this is negative, primers Mon340/348 which amplify a 289-nt fragment of

ORF1a are used. For genotyping, fragments of

ORF1a, ORF1b, and ORF2 are amplified by RT-PCR and sequenced.[29] A novel hanging drop single-tube nested RT-PCR method has recently been devised.[34] Recombinant antigens have been used to determine the seropreva-lence of astrovirus infections.[35] This is of little value for acute diagnosis.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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