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Fig. 1 Ligase chain reaction. The probes are annealed to template molecules in a head-to-tail fashion with the 3' end of one probe abutting the 5' end of the second probe. DNA ligase then joins the adjacent 3' and 5' ends to form a copy of one strand of the target. The copy is subsequently separated from the target by heat denaturation. Released ssDNA forms new templates for further probe binding. A second set of probes, complementary to the first, uses the duplicated strand, as well as the original target, as a template for ligation. (View this art in color at www.dekker.com.)

LCR was reported to be less affected by inhibitors in some specimens than PCR, LCR is subject to contamination that is at least as much of a problem as for PCR.[7] In particular, the inhibitory effect of phosphate on LCR can pose a problem,[17] and duplicate dilution analysis of negative specimens was recommended.[18]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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