Accreditation of the Medical Laboratory—ISO 15189/ISO 17025 / Mario Plebani 1

ACE Genotyping / Michael Rudnicki and Gert Mayer 7

Actinomyces spp. / Val Hall 12

Acute Myeloid Leukemia (AML)—Cytogenetic Detection of Selected Recurrent Chromosomal

Abnormalities / Hon Fong L. Mark, Robert A. Sokolic, and Yvonne Mark 17

Adeno-Associated Viral Vectors / Barrie J. Carter 25

Adenoviral Gene Therapy / Willemijn M. Gommans, Hidde J. Haisma, and

Marianne G. Rots 29

Alagille Syndrome / Michelle Hadchouel and Michele Meunier-Rotival 33

Allele-Specific Oligonucleotide Hybridization / Damian Labuda, Maja Krajinovic, and Stephane Bourgeois 38

Alpha-1-Antitrypsin Deficiency / Noor Kalsheker and Kevin Morgan 42

Alpha-Thalassemia / Renzo Galanello and Antonio Cao 47

Alzheimer's Disease, Familial, Early Onset / Beatriz Marcheco Teruel 52

Aminoglycoside-Induced Deafness / Mustafa Tekin 57

Amplification Refractory Mutation System PCR / Joannis E. Vamvakopoulos 62

Amplified Fragment Length Polymorphism (AFLP)—Application for DNA Fingerprinting /

G. Ripabelli and J. McLauchlin 68

Angelman Syndrome / Milen Velinov and Edmund C. Jenkins 73

Arylamine N-Acetyltransferase Gene Polymorphism / Neville J. Butcher and

Rodney F. Minchin 79

Astrovirus / C. Anthony Hart and Rachel L. Hart 84

Automated DNA Sequencing / Alexandre Izmailov 88

Automated Nucleic Acid Extraction / Juergen Loeffler, Kathrin D. Schmidt,

Holger Hebart, and Hermann Einsele 93

Barriers to Integrating New Genetic Knowledge into Daily Medical Practice /

Carol Isaacson Barash 97

Basal Cell Nevus Syndrome / A. S. High 102

Bead-Based Technology in Nucleic Acid Testing / Mario C. O. Probst 107

Becker Muscular Dystrophy / Masafumi Matsuo 111

Beta-Thalassemias / Antonio Cao and Renzo Galanello 114

Biosensors—DNA-Based Sensor Technology / Wenonah A. Vercoutere 119

Bladder Cancer—Identification of Bladder Cancer Biomarkers by Microarrays /

Marta Sanchez-Carbayo 124

Blood Group Genotyping / Lilian Castilho 130

Blotting Techniques / Philip Lewis White and Sam Hibbitts 135

Molecular Detection and Epidemiological Typing of Bordetella pertussis and

Bordetella parapertussis / Johanna Mäkinen, Matti K. Viljanen, Jussi Mertsola, and Qiushui He 140

Borrelia spp. / Maurice M. Exner 146

Branched DNA Signal Amplification / R. Stefan Ross 150

Brucella spp. / Vito G. DelVecchio and Cesar V. Mujer 155

Brugada Syndrome / Robert Roberts and Ramon Brugada 160

Burkholderia pseudomallei / Sumalee Tungpradabkul 165

Butyrylcholinesterase Gene Polymorphism / Weidong Liu and Toshikazu Hada 170

CADASIL / Marie Magdeleine Ruchoux and Michel Franck 174

Calicivirus / C. Anthony Hart and A. D. Radford 180

Campylobacter spp. / Kieran Jordan, Caroline Murphy, and Cyril Carroll 185

Cancer Chemotherapy—Overview on the Clinical Impact of Polymorphisms in Metabolizing Enzymes / Ikuo Sekine and Nagahiro Saijo 189

Cancer Chemotherapy—Polymorphisms in DPD, TPMT, UGT1A1 / Thomas Szekeres,

Alexander C. Haushofer, and Monika Fritzer-Szekeres 194

Candida spp. / Philip Lewis White and Sam Hibbitts 199

Capillary Electrochromatography / Vladislav Kahle 206

Capillary Electrophoresis / Ronald J. A. Trent, Melody Caramins, and Bing Yu 210

Cardiomyopathy, Dilated (Familial) / Dagmar I. Keller and Gisele Bonne 216

Cardiomyopathy, Hypertrophic (Familial) / Dagmar I. Keller and Lucie Carrier 221

Certified Reference Materials for Genetic Testing / David E. Barton,

Christoph Klein, and Glyn Stacey 226

Chemical Cleavage of Mismatch (CCM) / Neil V. Whittock and Louise Izatt 232

Chlamydia pneumoniae, Chlamydophila pneumoniae / Sven Poppert,

Udo Reischl, and Andreas Essig 237

Chlamydia trachomatis / Hsi Liu 243

Chromogenic In Situ Hybridization (CISH) / Bae-Li Hsi 248

Chronic Myelogenous Leukemia—Molecular Diagnosis and Monitoring / Jo-Anne Vergilio and Adam Bagg 252

Clarithromycin Resistance Associated Gene Mutations / Sabine Pereyre, Armelle Menard,

Cecile Bebear, and Francis Megraud 259

Clostridium botulinum / Miia Lindström, Mari Nevas, and Hannu Korkeala 266

Clostridium difficile / Michel G. Bergeron and Maurice Boissinot 270

Clostridium perfringens / Derek J. Fisher, James G. Smedley, III, Qiyi Wen, and Bruce A. McClane 275

Colony PCR / Gehua Wang 280

Commercial DNA Extraction Kits / Stuart C. Clarke 283

Comparative Genomic Hybridization (CGH) / Brynn Levy 287

Competitive PCR / M. Pazzagli, C. Orlando, and P. Pinzani 292

Corynebacterium diphtheriae—Molecular Detection of Diphtheria Toxin /

Jennifer K. Spinler and Randall K. Holmes 297

Cowden Syndrome / Deborah J. Marsh and Roberto T. Zori 301

Creutzfeldt-Jacob Disease M129V Polymorphism / Thomas R. Kreil, Hans Peter Schwarz, and Friedrich Dorner 305

Cryptosporidium parvum / Rebecca A. Guy and Paul A. Horgen 309

CYP1A2*1F and CYP2D6 Genotyping by Real-Time PCR / Barbara Messner and Werner Steimer 315

Cystic Fibrosis—Mutation Detection by Microarrays / Wayne W. Grody and Carolyn Sue Richards 319

Cytochrome P450 Enzyme Genotyping—Clinical Applications /

Magnus Ingelman-Sundberg 323

Denaturing Gradient Gel Electrophoresis (DGGE) /

Luciana Cresta de Barros Dolinsky 328

Differential Display (DD) Analysis / Farid E. Ahmed 331

Differential Sequencing by Mass Spectrometry / Christian Jurinke, Christiane Hönisch, and Dirk van den Boom 336

Disease Gene Patents / Jon F. Merz 342

Disposable Electrochemical DNA Biosensors / Kagan Kerman and Mehmet Ozsoz 346

DNA-Binding Fluorophores / Mikael Kubista, Jonas Karlsson, Martin Bengtsson,

Neven Zoric, and Gunar Westman 351

DNA Extraction from Mummified Tissues / Yayoi Sato 356

DNA Storage / C. William Kilpatrick 360

DNannotator—Annotation Software Tool Kit for Regional Genomic Sequences /

Chunyu Liu 364

Duchenne Muscular Dystrophy / Masafumi Matsuo 370

Echinococcus granulosus and Echinococcus multilocularis—Molecular Diagnosis /

Donald P. McManus 374

Ehlers-Danlos Syndrome / Anne De Paepe, Paul Coucke, and Fransiska Malfait 380

Electrospray Tandem Mass Spectrometry for Newborn Screening / Yosuke Shigematsu 386

Entamoeba histolytica, Entamoeba dispar / Patrick Gonin 391

Epstein-Barr Virus (EBV) / Fritz Schwarzmann, Hans Wolf, and Hans Helmut Niller 395

Escherichia coli Serotypes (Enterohemorrhagic)—Detection by Multiplex PCR /

Peter C. H. Feng and Steven R. Monday 400

ESTAnnotator—A Tool for High-Throughput EST Annotation / Agnes Hotz-Wagenblatt and Thomas Hankeln 404

Ethical Issues Related to Genetic Testing / Carol Isaacson Barash 409

Fabry Disease / Catherine Caillaud 414

Facioscapulohumeral Muscular Dystrophy / Meena Upadhyaya and

David N. Cooper 419

Factor IX Deficiency / Marian Hill and Gerry Dolan 426

Factor X Deficiency / Fabrizio Vianello 431

Factor XI Deficiency / Pierre Hopmeier 437

Familial Adenomatous Polyposis (FAP) Syndrome / Nader Hanna, Shaun McKenzie, and Heather Pierce 442

Fanconi Anemia / Hans Joenje, Gerard Pals, and Christian Michel Zwaan 447

FISH—An Overview / Yao-Shan Fan 452

FISH—Banding Methods and Clinical Applications / Thomas Liehr 457

FISH—Blood and Bone Marrow / Maria D. Odero, Idoya Lahortiga, Isabel Zudaire, and Maria J. Calasanz 461

FISH—Centromere-Specific Multicolor FISH / Thomas Liehr 468

FISH—Clinical Applications of 24-Color FISH / Adewale Adeyinka and

Syed M. Jalal 471

FISH—Detection of Individual Radiosensitivity / Alma Kuechler and Thomas Liehr 474

FISH—Human Sperm Cells / Renee H. Martin 478

FISH—Q-FISH for Telomere Length Measurement / Predrag Slijepcevic 482

FISH—Spectral Karyotyping and Multiplex FISH / Mario A. J. A. Hermsen,

Rhona M. Anderson, Gerrit A. Meijer, and Natalia D. Sumption 486

Fluorescence Resonance Energy Transfer (FRET) / Dino A. De Angelis 491

Fluoroquinolone Resistance-Associated Gene Mutations in Streptococcus pneumoniae /

George G. Zhanel and Heather J. Smith 496

Forensic DNA Samples—Collection and Handling / Mark Benecke 500

Forensic DNA Typing—Y Chromosome / Lluis Quintana-Murci 505

Forensic Identification—An Overview on Molecular Diagnostic Technology /

Adrian Linacre and Yvonne E. Cruickshank 509

Fragile X Syndrome / James N. Macpherson 513

Gaucher Disease / Deborah Elstein and Ari Zimran 519

Gene Dosage—Multiplex Probe Ligation and Amplification / Anders O. H. Nygren and Jan P. Schouten 524

Giardia lamblia / Enedina Jimenez-Cardoso 529

Glutathione S-Transferase Genotype / Richard C. Strange and Anthony A. Fryer 536

Glycogen Storage Diseases / Yuan-Tsong Chen and Deeksha Sarihyan Bali 543

HAART Therapy—Antiretroviral Resistance Genotyping / Kristel Van Laethem and Anne-Mieke Vandamme 550

Haemophilus ducreyi / Sylvia M. Bruisten 555

Handheld Nucleic Acid Analyzer / James A. Higgins 560

Haploid Analysis (Monosomal Hybrid Technique) / Robert E. Pyatt 565

HBV Quantification / Annemarie Berger 570

HCV Genotyping / Alexander C. Haushofer and Harald H. Kessler 574

HCV Quantification / Alexander C. Haushofer and Harald H. Kessler 579

Helper-Dependent Adenoviral Vectors / Philip Ng 584

Hemoglobinopathies, Structural / Lewis P. Rubin 589

Hereditary Breast and Ovarian Cancer Syndrome / Nader Hanna and Heather Pierce 594

Hereditary Diffuse Gastric Cancer Syndrome / Nader Hanna 600

Hereditary Hemochromatosis / Ronald T. Acton and James C. Barton 605

Hereditary Hemorrhagic Telangiectasia (Rendu-Osler-Weber Disease) / Carlo Sabba,

Anna Cirulli, and Ginevra Guanti 612

Hereditary Nonpolyposis Colorectal Cancer (HNPCC) Syndrome / Shaun McKenzie,

Heather Pierce, and Nader Hanna 617

Heteroduplex Analysis (HA) / Philip L. Beales 622

HLA Typing by PCR-Based Techniques / J. -M. Tiercy 628

HLA-DQA1 Typing Using DNA Microarray / Dat D. Dao and Laurie R. Lewis 633

HPV Typing—Comparison of Different Molecular Assays / Antoinette A. T. P. Brink,

Chris J. L. M. Meijer, Adriaan J. C. van den Brule, and Peter J. F. Snijders 640

HTLV-1 / Shimeru Kamihira 646

Human Herpesvirus (HSV) 1 and 2—Resistance Testing / Florence Morfin,

Emilie Frobert, and Danielle Thouvenot 651

Human Herpesvirus 6 and 7 / David H. Dockrell 656

Molecular Diagnosis of HIV-1 and HIV-2 / John R. Clarke 661

HyBeaconTM Probes / David John French 666

Immunoisolated Cells / Dwaine F. Emerich and Christopher G. Thanos 671

Introduction of Molecular Genetics and Genomics Into Clinical Practice /

Ronald J. A. Trent, Bing Yu, and Melody Caramins 676

Invader® Assay / Stephen Day and Andrea Mast 682

Inverse PCR / Philip Lewis White and Sam Hibbitts 687

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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