F2 Isoprostanes

Prostaglandin F2-like compounds (F2-isoprostanes) are formed by nonenzymatic free radical induced peroxidation of arachidonic acid (87) (Figure 10.5). These compounds are formed in phospholipids and then cleaved and released into the circulation before excretion in the urine as free isoprostanes (87). They have been extensively used as novel markers of endogenous lipid peroxidation and potential mediators of oxidant injury (88,89). Elevated levels of F2-isoprostanes have been seen in many diseases including diabetes, myocardial infraction, atherosclerosis, Alzheimer's, and in hepatic toxicity (90-94). Detection and quantification of F2-isoprostanes in biological samples including blood serum and plasma is now used as a tool in assessing oxidative stress status (95). The most common isoprostane formed is 8-isoprostaglandin F2, which can be detected accurately and easily by using GC/MS or by HPLC/MS (95-97). The longer analysis time required for GC/MS and HPLC/MS has resulted in the development of many immunochemical assays (91,98). However, these immunochemical methods suffer crossreactivity from biological prostaglandins due to their close structural similarity (91).

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Supplements For Diabetics

Supplements For Diabetics

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