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Figure 3. Searching for new methylated genes by combining cDNA microarray technology with 5-aza-2-deoxycytidine treatment. A. Measurement of 5-methylcytosine content by HPCE in mouse skin cancer cell lines treated and untreated with 5-aza-2-deoxycytidine. Results are expressed as the mean ± SD. B. representative blocks of a MouseChip array showing overexpression (red circles) of igfbp3 gene in CarC cell line relative to MCA3D. C. Summary of the methylation-specific PCR (MSP) and bisulfite sequencing analyses of the CpG island methylation status of five positive genes in the DNA microarray. D. Schematic representation of the methylation status of the CpG islands of some of the candidate genes in several mouse skin cancer cell lines obtained by bisulfite genomic sequencing. E. Example of the MSP analysis of some of the candidate genes identified. F. Example of the RT-PCR analysis of the Ache, and prdx1 genes. Restoration of gene expression is observed in PAM212 cell lines treated with Aza when compared with the untreated ones. P-actin expression is shown as an internal control.

10 Ways To Fight Off Cancer

10 Ways To Fight Off Cancer

Learning About 10 Ways Fight Off Cancer Can Have Amazing Benefits For Your Life The Best Tips On How To Keep This Killer At Bay Discovering that you or a loved one has cancer can be utterly terrifying. All the same, once you comprehend the causes of cancer and learn how to reverse those causes, you or your loved one may have more than a fighting chance of beating out cancer.

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