Edited by

Kursad Turksen

Ottawa Health Research Institute Ottawa, Ontario, Canada

Humana Press ^ Totowa, New Jersey

© 2006 Humana Press Inc. 999 Riverview Drive, Suite 208 Totowa, New Jersey 07512


All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise without written permission from the Publisher. Methods in Molecular Biology™ is a trademark of The Humana Press Inc.

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Production Editor: Jennifer Hackworth

Cover design by Patricia F. Cleary

Cover illustration: Figure 2D from Chapter 7 in Vol. 1, "Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture," by Jennifer Nichols and Qi-Long Ying.

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eISBN: 1-59745-037-5 ISSN: 1064-3745

Library of Congress Cataloging-in-Publication Data

Embryonic stem cell protocols / edited by Kursad Turksen.-- 2nd ed. v. ; cm. -- (Methods in molecular biology ; v. 329-330) Includes bibliographical references and index. Contents: v. 1. Isolation and characterization -- v. 2. Differentiation models.

ISBN 1-58829-498-6 (v. 1 : alk. paper) -- ISBN 1-58829-784-5 (v. 2 : alk. paper)

1. Stem cells--Laboratory manuals. 2. Embryos--Laboratory manuals. [DNLM: 1. Stem Cells. 2. Embryo Research. 3. Models, Animal. QU 325 N812 2006] I. Turksen, Kursad. II. Series: Methods in molecular biology (Clifton, N.J.) ; v. 329-330. QH588.S83E46 2006 616'.02774--dc22

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