Volume 2 Differentiation Models

1 Neural Stem Sphere Method: Induction of Neural Stem Cells and Neurons by Astrocyte-Derived Factors in Embryonic Stem

Cells In Vitro Takashi Nakayama and Nobuo Inoue

2 Generation and Characterization of Oligodendrocytes From

Lineage-Selectable Embryonic Stem Cells In Vitro Nathalie Billon, Christine Jolicoeur, and Martin Raff

3 Derivation and Characterization of Neural Cells From Embryonic Stem

Cells Using Nestin Enhancer Nibedita Lenka

4 Optimized Neuronal Differentiation of Murine Embryonic

Stem Cells: Role of Cell Density Matthew T. Lorincz

5 Generation of Inner Ear Cell Types From Embryonic Stem Cells Marcelo N. Rivolta, Huawei Li, and Stefan Heller

6 Derivation of Epidermal Colony-Forming Progenitors

From Embryonic Stem Cell Cultures Tammy-Claire Troy and Kursad Turksen

7 Directing Epidermal Fate Selection by a Novel Co-Culture System Tammy-Claire Troy and Kursad Turksen

8 In Vitro Generation of T Lymphocytes From Embryonic Stem Cells Renée F. de Pooter, Thomas M. Schmitt, and Juan Carlos Zuniga-Pflucker

9 The Role of Hex in Hemangioblast and Hematopoietic Development Rebecca J. Chan, Robert Hromas, and Mervin C. Yoder

10 Generation of Osteoblasts and Chondrocytes

From Embryonic Stem Cells Jitsutaro Kawaguchi

11 Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures Nicole L. Woll and Sarah K. Bronson

12 Generation of Chondrocytes From Embryonic Stem Cells Jaspal Singh Khillan

13 Derivation and Characterization of Chondrocytes From

Embryonic Stem Cells In Vitro Jan Kramer, Gunnar Hargus, and Jürgen Roh wedel

14 Generation and Characterization of Cardiomyocytes Under

Serum-Free Conditions Cornelia Gissel, Michael Xavier Doss, Rita Hippler-Altenburg,

Jürgen Hescheler, and Agapios Sachinidis

15 Analysis of Arrhythmic Potential of Embryonic Stem

Cell-Derived Cardiomyocytes Lijuan L. Shang, Samuel C. Dudley, Jr., and Arnold E. Pfahnl

16 Derivation and Characterization of Alveolar Epithelial Cells

From Murine Embryonic Stem Cells In Vitro Ali Samadikuchaksaraei and Anne E. Bishop

17 Derivation and Characterization of Thyrocyte-Like Cells

From Embryonic Stem Cells In Vitro Reigh-Yi Lin and Terry F. Davies

18 Derivation and Characterization of Gut-Like Structures

From Embryonic Stem Cells Takatsugu Yamada and Yoshiyuki Nakajima

19 Formation of Gut-Like Structures In Vitro From Mouse

Embryonic Stem Cells Shigeko Torihashi

20 In Vitro Derivation and Expansion of Endothelial Cells

From Embryonic Stem Cells Kara E. McCloskey, Steven L. Stice, and Robert M. Nerem

21 Differentiation of Mouse Embryonic Stem Cells Into Endothelial

Cells: Genetic Selection and Potential Use In Vivo Clotilde Gimond, Sandrine Marchetti, and Gilles Pages

22 Integrins and Vascular Development in Differentiated

Embryonic Stem Cells In Vitro Sheila E. Francis

23 TGF-ß Signaling in Embryonic Stem Cell-Derived Endothelial Cells Tetsuro Watabe, Jun K. Yamashita, Koichi Mishima, and Kohei Miyazono

24 The Role of the Adapter Protein SHB in Embryonic

Stem Cell Differentiation Into the Pancreatic ß-cell and Endothelial Lineages Johan Saldeen, Nina Agren, Björn Akerblom, Lingge Lu,

Bashir Adem, Lukasz S§dek, and Vitezslav Kriz

25 In Vitro Differentiation of Embryonic Stem Cells Into the Pancreatic Lineage Przemyslaw Blyszczuk and Anna M. Wobus

26 Derivation and Characterization of Hepatocytes From Embryonic

Stem Cells In Vitro Shigeaki Ishizaka, Yukiteru Ouji, Masahide Yoshikawa, and Kazuki Nakatani

27 Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro Xing Zhao, Jianuo Liu, and Iqbal Ahmad

28 Derivation and Characterization of Lentoid Bodies and Retinal

Pigment Epithelial Cells From Monkey Embryonic Stem Cells In Vitro Masayo Takahashi and Masatoshi Haruta

29 Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-

Dimensional Collagen Matrix Silvia Sihui Chen, Roberto P. Revoltella, Joshua Zimmerberg, and Leonid Margolis

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