The author is indebted to his collaborators at ZEBET in the BfR; Dr. Barbara Grune, Helena Kandarova, Dr. Manfred Liebsch, Dr. Andrea Seiler, and Dr. Richard Vogel, without whom ZEBET would not have reached its international reputation as one of the leading centers devoted to the 3Rs, as proposed by William Russell and Rex Burch. Our studies at ZEBET have depended on generous funding from ECVAM, the EU Centre for Validation, and the BMBF, the National Department for Research and Technology of the Federal Government of Germany.

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